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ie ming shih  (Addgene inc)


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    Structured Review

    Addgene inc ie ming shih
    Ie Ming Shih, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ie+ming+shih/pcDNA6-ARID1A+(Plasmid+%2339311)/pmc13000123-349-45-50
    Average 93 stars, based on 19 article reviews
    ie ming shih - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.
    Article Snippet: .. 12 NATURE COMMUNICATIONS | (2021) 12:5337 | https://doi.org/10.1038/s41467-021-25622-3 | www.nature.com/naturecommunications Dario Vignali (Addgene plasmid # 52107)79. pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481)80. .. To generate retrovirus, retroviral transfer and envelope/packaging plasmid were transfected into HEK-293T Lenti-X cells (Takara Bio) with Transporter 5 transfection reagent (Polysciences) according to manufacturer’s protocol.

    Article Title: Targeting the TCA cycle through cuproptosis confers synthetic lethality on ARID1A-deficient hepatocellular carcinoma.
    Article Snippet: .. The pLenti-puro-ARID1A plasmid was a kind gift from Ie-Ming Shih (Addgene; #39478) and lentiviral production was performed as described above. ..

    Article Title: Discovery and characterization of anti-cancer peptides from a random peptide library.
    Article Snippet: .. FP: GATCC GCCACCATGCgccagattaaaatttggtttcagaaccgccgcatgaaatgg aaaaaaggcggcNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNcatca ccatcaccatcactgaG PLOS ONE | https://doi.org/10.1371/journal.pone.0293072 February 13, 2024 10 / 16 RP: AATTC tcagtgatggtgatggtgatgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNgccgccttttttccatttcatgcggcggttctgaaaccaaattttaa tctggcgCATGGTGGCG pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481) [55] psPAX1 was a gift from Didier Trono (Addgene plasmid # 12260) pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259) Briefly, lentivirus was produced by transfection of 10 μg of plenti-puro-15mer-PA clone, 10 μg of psPAX and 5 μg of VSVG plasmids into 293T cells by TurboFectin 8.0 transfection reagent at the ration of 1:3 (DNA: Reagent) as per the manufacturer’s protocol. ..

    Article Title: Cardiomyocyte proliferation is suppressed by ARID1A-mediated YAP inhibition during cardiac maturation.
    Article Snippet: On day 4, luciferase activity was measured using Dual-Luciferase® Reporter Assay System (Promega, # E1910) and the Berthold Centro LB 960 luminometer according to manufacturers instructions. .. pcDNA6-ARID1A was a gift from Ie-Ming Shih (Addgene plasmid # 39311). .. 8xGTIIC-luciferase construct was a gift from Stefano Piccolo (Addgene plasmids # 34615).

    Article Title: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.
    Article Snippet: The KO was confirmed by Sanger DNA sequencing (Microsynth) using the following primer 5ʹ-AGATGCAGGGGAGGTCCTGAAA-3ʹ. .. CD317 Overexpression in Human Glioma Cells The CD317 coding sequence was synthesized by Genscript (Leiden, Netherlands) and cloned into pLenti-puro. pLentipuro was a gift from Ie-Ming Shih (Addgene plasmid #39481). ..

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors
    Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites. .. Sequences were verified by Sanger sequencing and are provided as supplementary information. pLX304 was a gift from David Root (Addgene plasmid # 25890; http://n2t.net/addgene : 25890; RRID:Addgene 25890). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481; http://n2t.net/addgene : 39481; RRID:Addgene 39481). .. HCT116 and HEK293T cells were cultured in RPMI or DMEM (Invitrogen) with 10% Fetal Bovine Serum (Hyclone).

    Article Title: Targeting peroxiredoxin 2 prevents hepatocarcinogenesis in metabolic liver disease
    Article Snippet: .. The authors 17 thank the Centre de Ressources Biologiques (CRB)/Biological Resource Centre, Strasbourg, 18 France, for the management of patient-derived liver tissues and access to clinical information 19 and the Animal Experimentation Platform Infection and Cancer (ÆPIC) (University of 20 Strasbourg, ITM Inserm UMR_S1110, Strasbourg, France) for the management of animal 21 experiments. pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481; 22 RRID:Addgene_39481). ..

    Article Title: Huntingtin (HTT) interactome in regulation of DNA repair/remodeling and RNA processing pathways
    Article Snippet: .. The following plasmids were used: pEF-BOS-T7-CA150 was a gift from Mariano Garcia-Blanco (plasmid # 21924; Addgene; http://n2t.net/addgene:21924 ; RRID:Addgene_21924) ( ); pCMV5 BRGI-Flag was a gift from Joan Massague (plasmid # 19143; Addgene; http://n2t.net/addgene:19143 ; RRID: Addgene_19143) ( ); and pcDNA6-ARID1A was a gift from Ie-Ming Shih (plasmid # 39311; Addgene; http://n2t.net/addgene:39311 ; RRID:Addgene_39311) ( ). .. We have validated anti-phospho DNA-PKcs (S2056) antibody (#18192; Abcam) and total DNA-PKcs (E6U3A) antibody (#38168; Cell Signaling Technology) in our ISPNs using DNA-PKcs knockdown to confirm specificity ( ): ISPNs were transfected at a final concentration of 1.5uM with siRNA to DNA-PKcs (Santa Cruz Biotechnology) or with scrambled control siRNA.

    Produced:

    Article Title: Discovery and characterization of anti-cancer peptides from a random peptide library.
    Article Snippet: .. FP: GATCC GCCACCATGCgccagattaaaatttggtttcagaaccgccgcatgaaatgg aaaaaaggcggcNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNcatca ccatcaccatcactgaG PLOS ONE | https://doi.org/10.1371/journal.pone.0293072 February 13, 2024 10 / 16 RP: AATTC tcagtgatggtgatggtgatgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNgccgccttttttccatttcatgcggcggttctgaaaccaaattttaa tctggcgCATGGTGGCG pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481) [55] psPAX1 was a gift from Didier Trono (Addgene plasmid # 12260) pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259) Briefly, lentivirus was produced by transfection of 10 μg of plenti-puro-15mer-PA clone, 10 μg of psPAX and 5 μg of VSVG plasmids into 293T cells by TurboFectin 8.0 transfection reagent at the ration of 1:3 (DNA: Reagent) as per the manufacturer’s protocol. ..

    Transfection:

    Article Title: Discovery and characterization of anti-cancer peptides from a random peptide library.
    Article Snippet: .. FP: GATCC GCCACCATGCgccagattaaaatttggtttcagaaccgccgcatgaaatgg aaaaaaggcggcNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNcatca ccatcaccatcactgaG PLOS ONE | https://doi.org/10.1371/journal.pone.0293072 February 13, 2024 10 / 16 RP: AATTC tcagtgatggtgatggtgatgNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNgccgccttttttccatttcatgcggcggttctgaaaccaaattttaa tctggcgCATGGTGGCG pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481) [55] psPAX1 was a gift from Didier Trono (Addgene plasmid # 12260) pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259) Briefly, lentivirus was produced by transfection of 10 μg of plenti-puro-15mer-PA clone, 10 μg of psPAX and 5 μg of VSVG plasmids into 293T cells by TurboFectin 8.0 transfection reagent at the ration of 1:3 (DNA: Reagent) as per the manufacturer’s protocol. ..

    Over Expression:

    Article Title: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.
    Article Snippet: The KO was confirmed by Sanger DNA sequencing (Microsynth) using the following primer 5ʹ-AGATGCAGGGGAGGTCCTGAAA-3ʹ. .. CD317 Overexpression in Human Glioma Cells The CD317 coding sequence was synthesized by Genscript (Leiden, Netherlands) and cloned into pLenti-puro. pLentipuro was a gift from Ie-Ming Shih (Addgene plasmid #39481). ..

    Sequencing:

    Article Title: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.
    Article Snippet: The KO was confirmed by Sanger DNA sequencing (Microsynth) using the following primer 5ʹ-AGATGCAGGGGAGGTCCTGAAA-3ʹ. .. CD317 Overexpression in Human Glioma Cells The CD317 coding sequence was synthesized by Genscript (Leiden, Netherlands) and cloned into pLenti-puro. pLentipuro was a gift from Ie-Ming Shih (Addgene plasmid #39481). ..

    Article Title: A simple method for analyzing competitive growth of multiple cell types in xenograft tumors
    Article Snippet: A PacI-XhoI fragment from each of the six pLenti-puro tag vectors was inserted into a modified pLX304 vector (Addgene 25890; [ ]), removing the CMV promoter and any polylinker cloning sites. .. Sequences were verified by Sanger sequencing and are provided as supplementary information. pLX304 was a gift from David Root (Addgene plasmid # 25890; http://n2t.net/addgene : 25890; RRID:Addgene 25890). pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481; http://n2t.net/addgene : 39481; RRID:Addgene 39481). .. HCT116 and HEK293T cells were cultured in RPMI or DMEM (Invitrogen) with 10% Fetal Bovine Serum (Hyclone).

    Synthesized:

    Article Title: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.
    Article Snippet: The KO was confirmed by Sanger DNA sequencing (Microsynth) using the following primer 5ʹ-AGATGCAGGGGAGGTCCTGAAA-3ʹ. .. CD317 Overexpression in Human Glioma Cells The CD317 coding sequence was synthesized by Genscript (Leiden, Netherlands) and cloned into pLenti-puro. pLentipuro was a gift from Ie-Ming Shih (Addgene plasmid #39481). ..

    Clone Assay:

    Article Title: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.
    Article Snippet: The KO was confirmed by Sanger DNA sequencing (Microsynth) using the following primer 5ʹ-AGATGCAGGGGAGGTCCTGAAA-3ʹ. .. CD317 Overexpression in Human Glioma Cells The CD317 coding sequence was synthesized by Genscript (Leiden, Netherlands) and cloned into pLenti-puro. pLentipuro was a gift from Ie-Ming Shih (Addgene plasmid #39481). ..

    Infection:

    Article Title: Targeting peroxiredoxin 2 prevents hepatocarcinogenesis in metabolic liver disease
    Article Snippet: .. The authors 17 thank the Centre de Ressources Biologiques (CRB)/Biological Resource Centre, Strasbourg, 18 France, for the management of patient-derived liver tissues and access to clinical information 19 and the Animal Experimentation Platform Infection and Cancer (ÆPIC) (University of 20 Strasbourg, ITM Inserm UMR_S1110, Strasbourg, France) for the management of animal 21 experiments. pLenti-puro was a gift from Ie-Ming Shih (Addgene plasmid # 39481; 22 RRID:Addgene_39481). ..



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    Addgene inc ie ming shih
    Ie Ming Shih, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ie+ming+shih/pcDNA6-ARID1A+(Plasmid+%2339311)/pmc13000123-349-45-50
    Average 93 stars, based on 1 article reviews
    ie ming shih - by Bioz Stars, 2026-10
    93/100 stars
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